Organelle Proteomics

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ISBN-13:
9781588297792
Einband:
Gb
Erscheinungsdatum:
01.03.2008
Seiten:
422
Autor:
Delphine Pflieger
Gewicht:
850 g
Format:
244x162x30 mm
Serie:
432, Methods in Molecular Biology
Sprache:
Englisch
Beschreibung:

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. This is the first book that examines organelle proteomics in depth. Detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Also, devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, the book allows scientists to gain a vital and important understanding of organelle study.
1. - Analysis of organelles by on-line two-dimensional liquid chromatography - tandem mass spectrometry (2DLC-MS/MS) Edwin P. Romijn and John R. Yates III. Analysis of organelles, vesicles and protein machineries 2 - Purification and proteomic analysis of chloroplasts and their sub-organellar compartmentsDaniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 3 - Proteomic analysis of the stacked Golgi complex
Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu 4 - Purification of Saccharomyces cerevisiae mitochondria by zone-electrophoresis in a free flow device (ZE-FFE)
Hans Zischka, Norbert Kinkl, Ralf J. Braun and Marius Ueffing 5 - Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis
Claire Lemaire and Geneviève Dujardin 6 - Mitochondrial proteomics: Analysis of a whole mitochondrial extract with two-dimensional electrophoresis Thierry Rabilloud. 7 - Purification and proteomic analysis of the mouse liver mitochondrial inner membrane
Sandrine Da Cruz and Jean-Claude Martinou 8 - Subcellular Fractionation and Proteomics of Nuclear Envelopes Laurence Florens, Nadia Korfali and Eric C. Schirmer. 9 - Purification and proteomic analysis of the nuclear insoluble protein fraction
Tsuneyoshi Horigome, Kazuhiro Furukawa and Kohei Ishii 10 - Preparation Methods of Human Metaphase Chromosomes for Their Proteome Analysis.
Kiichi Fukui and Hideaki Takata 11 - Purification and proteomic analysis of plant plasma membranes
Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom and Christer Larsson 12 - Protocol to enrich and analyze plasma membrane proteins from frozen tissue
Jacek R. Wisniewski 13 - Proteomic characterization of membrane proteintopology
Adele R. Blackler and Christine C. Wu 14 - Free Flow Isoelectric Focussing (FF-IEF): A method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes
Markus Islinger and Gerhard Weber 15 - Use of gas-phase-fractionation to increase protein identifications: application to the peroxisome
Jacob Kennedy and Eugene C. Yi 16 - Purification and proteomic analysis of lysosomal integral membrane proteins
Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan. 17 - Affinity purification of soluble lysosomal proteins for mass spectrometric identification
Sylvie Kieffer-Jaquinod, Agnès Chapel, Jérôme Garin and Agnès Journet 18 - Purification and proteomic analysis of synaptic vesicles
Holly D. Cox and Charles M. Thompson. 19 - Purification and proteomic analysis of pancreatic zymogen granule membranes
Xuequn Chen and Philip C. Andrews 20 - Isolation and proteomic analysis of Chlamydomonas centrioles
Lani C. Keller and Wallace F. Marshall. 21 - Purification and proteomic analysis of 20S proteasomes from human cells
Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat, and Odile Burlet-Schiltz. 22 - Characterization of E. coli ribosomal particles: combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography - tandem mass spectrometry.
Isabelle Iost, Julie Charollais, Joëlle Vinh and Delphine Pflieger Proteins identified in the organelle sample: true components or contaminants? 23 - Assessment of organelle purity using antibodies and specific assays: the example of the chloroplast envelope
Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 24 - Identifying bona fide components of an organelle by isotope coded labelling of
Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

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