Neutrophil Methods and Protocols

 Book
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ISBN-13:
9781588297884
Einband:
Book
Erscheinungsdatum:
02.08.2007
Seiten:
564
Autor:
Gary M. Bokoch
Gewicht:
999 g
Format:
235x155x36 mm
Sprache:
Englisch
Beschreibung:

Provides neutrophil protocols that are broadly applicable to leukocyte biology
NeutrophilsMethods and Protocols
Edited by
Mark T. Quinn
Frank R. DeLeo
Gary M. Bokoch
Contents
Dedication i
Preface ii
Contributors v
PART I: NEUTROPHILS: AN OVERVIEW
1. The Role of Neutrophils in the Immune System: An Overview
Harry L. Malech
PART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION
2. Isolation of Human Neutrophils From Venous Blood
William M. Nauseef
3. Neutrophil Isolation From Non-Human Species
Daniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn
4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
Lene Udby and Niels Borregaard
PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS
5. Rac and Rap GTPase Activation Assays
Ulla G. Knaus, Alison Bamberg, and Gary M. Bokoch
6. Measurement of Phospholipid Metabolism in Intact Neutrophils
Susan Sergeant and Linda C. McPhail
7. Analysis of Protein Phosphorylation in Human Neutrophils
Jamel El-Benna and Pham My-Chan Dang
8. Mitogen-Activated Protein Kinase Assays
Martine Torres
9. Transduction of Proteins into Intact Neutrophils
Tieming Zhao and Gary M. Bokoch
10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
Esther J. Hillson, Sharon Dewitt, and Maurice B. Hallett
11. Analysis of Electrophysiological Properties and Responses of Neutrophils
Deri Morgan and Thomas E. DeCoursey
12. Analysis of Neutrophil Apoptosis
Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. Hart
PART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS
13. Optical and Fluorescence Detection of Neutrophil Integrin Activation
Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon
14. Analysis of Neutrophil Polarization and Chemotaxis
Mary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher
15. Quantifying and Localizing Actin Free Barbed Ends in Neutrophils
Michael Glogauer
16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda
17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
Nancy A. Louis, Eric Campbell, and Sean P. Colgan
PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY
18. Immunofluorescence and Confocal Microscopy of Neutrophils
Lee-Ann H. Allen
19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
Benjamin E. Steinberg and Sergio Grinstein
20. Analysis of Neutrophil Membrane Traffic During Phagocytosis
Per Lönnbro, Pontus Nordenfelt, and Hans Tapper
21. Analysis of Neutrophil Bactericidal Activity
Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton
22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
Per Follin and Claes Dahlgren
PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES
23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes
Claes Dahlgren, Anna Karlsson, and Johan Bylund
24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez
25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly
Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick
26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis
This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.

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